Chemical Sciences: A Manual for CSIR-UGC National Eligibility Test for Lectureship and JRF/Probe electrospray ionization

Probe electrospray ionization (PESI) is a modified version of conventional electrospray ionization (ESI), where the capillary for sample solution transferring is replaced by a solid needle with a sharp tip. PESI was first introduced by Kenzo Hiraoka et al. in 2007 Hiraoka K.; Nishidate K.; Mori K.; Asakawa D.; Suzuki S. Development of probe electrospray using a solid needle Rapid Communications in Mass Spectrometry 2007 21 3139–3144 10.1002/rcm.3201. Compared with conventional electrospray ionization, high salt tolerance and direct sampling and low sample consumption can be found with PESI. Of course, PESI is not a continuous process at all. Normally, the needle for sampling and spraying can be driven up and down under a frequency of 3-5 Hz. References