Chemical Sciences: A Manual for CSIR-UGC National Eligibility Test for Lectureship and JRF/Peptide sequence tag

A peptide sequence tag is a piece of information about a peptide obtained by tandem mass spectrometry that can be used to identify this peptide in a protein database.Hardouin J Protein sequence information by matrix-assisted laser desorption/ionization in-source decay mass spectrometry Mass spectrometry reviews 26 5 672–82 2007 17492750 10.1002/mas.20142Shadforth I, Crowther D, Bessant C Protein and peptide identification algorithms using MS for use in high-throughput, automated pipelines Proteomics 5 16 4082–95 2005 16196103 10.1002/pmic.200402091Mørtz E, O'Connor PB, Roepstorff P, Kelleher NL, Wood TD, McLafferty FW, Mann M Sequence tag identification of intact proteins by matching tanden mass spectral data against sequence data bases Proc. Natl. Acad. Sci. U.S.A. 93 16 8264–7 1996 8710858 10.1073/pnas.93.16.8264 Mass spectrometry In general, peptides can be identified by fragmenting them in a mass spectrometer. For example, during collision-induced dissociation peptides collide with a gas within the mass spectrometer and break into pieces at their peptide bonds. The resulting fragment ions (called b-ions and y-ions) have mass differences corresponding to the residue masses of the respective amino acids. Thus, a tandem mass spectrum contains partial information about the amino acid sequence of the peptide. The peptide sequence tag approach, developed by Matthias Wilm and Matthias Mann at the EMBL,Mann M, Wilm M Error-tolerant identification of peptides in sequence databases by peptide sequence tags Anal. Chem. 66 24 4390–9 1994 7847635 10.1021/ac00096a002 uses this information to identify the peptide in a database. Briefly, a couple of masses are extracted from the spectrum in order to obtain the peptide sequence tag. This peptide sequence tag is a unique identifier of a specific peptide and can be used to find it in a database containing all possible peptide sequences. Peptide fragment notation thumb|right|300 px|Peptide fragmentation notation using the scheme of Roepstorff and Fohlman (1984). Roepstorff P, Fohlman J Proposal for a common nomenclature for sequence ions in mass spectra of peptides Biomed. Mass Spectrom. 11 11 601 1984 6525415 10.1002/bms.1200111109 A notation has been developed for indicating peptide fragments that arise from a tandem mass spectrum. Peptide fragment ions are indicated by a, b, or c if the charge is retained on the N-terminus and by x, y or z if the charge is maintained on the C-terminus. The subscript indicates the number of amino acid residues in the fragment. Prime symbols indicate the number of protons or hydrogens added to the fragment to form the observed ion. For example, y'' denotes the singly charged ion analogous to a protonated peptide, (y''')2+ is a doubly-charged ion analogous to a doubly-protonated peptide.Tang XJ, Thibault P, Boyd RK Fragmentation reactions of multiply-protonated peptides and implications for sequencing by tandem mass spectrometry with low-energy collision-induced dissociation Anal. Chem. 65 20 2824–34 1993 October 7504416 References