Chemical Sciences: A Manual for CSIR-UGC National Eligibility Test for Lectureship and JRF/Top-down proteomics

Top-down proteomics is a method of protein identification that uses an ion trapping mass spectrometer to store an isolated protein ion for mass measurement and tandem mass spectrometry analysis.Sze SK, Ge Y, Oh H, McLafferty FW Top-down mass spectrometry of a 29-kDa protein for characterization of any posttranslational modification to within one residue Proc. Natl. Acad. Sci. U.S.A. 99 4 1774–9 2002 11842225 10.1073/pnas.251691898 122269Kelleher NL Top-down proteomics Anal. Chem. 76 11 197A–203A 2004 15190879 10.1021/ac0415657 The name is derived from the similar approach to DNA seqencing.Smith CL, Cantor CR Evolving strategies for making physical maps of mammalian chromosomes Genome 31 2 1055–8 1989 2698822 Proteins are typically ionized by electrospray ionization and trapped in a Fourier transform ion cyclotron resonance (Penning trap)Bogdanov B, Smith RD Proteomics by FTICR mass spectrometry: top down and bottom up Mass spectrometry reviews 24 2 168–200 2005 15389855 10.1002/mas.20015 or quadrupole ion trap (Paul trap) mass spectrometer. Fragmentation for tandem mass spectrometry is accomplished by electron-capture dissociation or electron-transfer dissociation. References Bibliography Borchers CH, Thapar R, Petrotchenko EV, et al. Combined top-down and bottom-up proteomics identifies a phosphorylation site in stem-loop-binding proteins that contributes to high-affinity RNA binding Proc. Natl. Acad. Sci. U.S.A. 103 9 3094–9 2006 16492733 10.1073/pnas.0511289103 1413926 Han X, Jin M, Breuker K, McLafferty FW Extending top-down mass spectrometry to proteins with masses greater than 200 kilodaltons Science 314 5796 109–12 2006 17023655 10.1126/science.1128868 Whitelegge J, Halgand F, Souda P, Zabrouskov V Top-down mass spectrometry of integral membrane proteins Expert review of proteomics 3 6 585–96 2006 17181473 10.1586/14789450.3.6.585