Proteomics/Protein Separations- Electrophoresis/History of Electrophoresis

History of Electrophoresis 1930s First reports of the use of sucrose for gel electrophoresis. 1937 The official launching of Tiselius' moving boundary electrophoresis (MBE). 1939 Zone Electrophoresi 1950 Rich in introducing novel methodologies in zone electrophoresis (ZE) 1950 Agar Gel electrophoresis 1955 Introduction of starch gels, not very good separation 1957 Cellulose acetate Electrophoresis(CAc) by Kohn 1959 Introduction of acrylamide gels for 1st time (Raymond and Winstraub) Accuracy of control of parameters such as pore size and stability 1961 Electrophoresis in silica gel by Honnegar 1961IsoElectricFocusing by Svensson 1964 Electrophoresis in thin layer of sephadex beads by Fasella et.al 1964 Disc gel electrophoresis Ornstein and Davis 1969 Introduction of denaturing agents especially SDS separation of protein subunit (Beber and Osborn) 1970 Laemmli-T4 phage separated 28 components Mstacking gel + SDS 1975 2 Dimensional gels OFarrell isoelectric focussing then SDS gel electrophoresis. 1977 Sequencing gels Late 1970s agarose gels 1980sImmobilized pH gradients 1983 Pulsed field gel electrophoresis 1983 Capillary electrophoresis introduced References http://www.csupomona.edu/~nebuckley/Courses/Bio451/Lectures/Bio451Lecture4.htm Electrophoresis: the march of pennies, the march of dimes. Righetti PG.