Chemical Sciences: A Manual for CSIR-UGC National Eligibility Test for Lectureship and JRF/Hybrid mass spectrometer

thumb|right|200 px|Hybrid linear ion trap Fourier transform ion cyclotron resonance mass spectrometer A hybrid mass spectrometer is a device for tandem mass spectrometry that consists of a combination of two or more m/z separation devices of different types. Notation The different m/z separation elements of a hybrid mass spectrometer can be represented by a shorthand notation. The symbol Q represents a quadrupole mass analyzer, q is a radio frequency collision quadrupole, TOF is a time-of-flight mass spectrometer, B is a magnetic sector and E is an electric sector. Sector quadrupole A sector instrument can be combined with a collision quadrupole and quadrupole mass analyzer to form a hybrid instrument. Glish G. 1982 A new “hybrid” sector/quadrupole mass spectrometer for mass spectrometry/mass spectrometry International Journal or Mass Spectrometry and Ion Physics 41 157 10.1016/0020-7381(82)85032-8 McLuckey S Ridley T Cooks R A BEqQ configuration with a magnetic sector (B), electric sector (E), collision quadrupole (q) and m/z selection quadrupole (Q) have been constructed Schoen A. 1985 A hybrid BEQQ mass spectrometer International Journal of Mass Spectrometry and Ion Processes 65 125 10.1016/0168-1176(85)85059-X Amy J.W. Ciupek J.D. Cooks R.G. Dobberstein P. Jung G. Harrison A. 1986 A hybrid BEQQ mass spectrometer for studies in gaseous ion chemistry International Journal of Mass Spectrometry and Ion Processes 74 13 10.1016/0168-1176(86)85020-0 and an instrument with two electric sectors (BEEQ) has been described. Winger B. E. 1992 Hybrid BEEQ tandem mass spectrometer for the study of ion/surface collision processes Review of Scientific Instruments 63 5613 10.1063/1.1143391 Laue H. -J. Horning S. R. Julian R. K. Lammert S. A. Riederer D. E. Cooks R. G. Quadrupole time-of-flight thumb|right|250 px|Hybrid quadrupole time-of-flight mass spectrometer. A triple quadrupole mass spectrometer with the final quadrupole replaced by a time-of-flight device is known is a quadrupole time-of-flight instrument.Shevchenko A, Loboda A, Shevchenko A, Ens W, Standing KG MALDI quadrupole time-of-flight mass spectrometry: a powerful tool for proteomic research Anal. Chem. 72 9 2132–41 2000 May 10815976 10.1021/ac9913659Steen H, Küster B, Mann M Quadrupole time-of-flight versus triple-quadrupole mass spectrometry for the determination of phosphopeptides by precursor ion scanning J Mass Spectrom 36 7 782–90 2001 July 11473401 10.1002/jms.174 Such an instrument can be represented as QqTOF. Ion trap time-of-flight In an ion trap instrument, ions are trapped in a quadrupole ion trap and then injected into the TOF. The trap can be 3-DFountain ST, Lee H, Lubman DM Ion fragmentation activated by matrix-assisted laser desorption/ionization in an ion-trap/reflectron time-of-flight device Rapid Commun. Mass Spectrom. 8 5 407–16 1994 May 7517726 10.1002/rcm.1290080514 or a linear trap. Campbell J. M. 1998 A new linear ion trap time-of-flight system with tandem mass spectrometry capabilities Rapid Communications in Mass Spectrometry 12 1463 10.1002/(SICI)1097-0231(19981030)12:20<1463::AID-RCM357>3.0.CO;2-H Collings B. A. Douglas D. J. Linear ion trap and Fourier transform mass analyzers A linear ion trap combined with a Fourier transform ion cyclotron resonanceSyka JE, Marto JA, Bai DL, et al. Novel linear quadrupole ion trap/FT mass spectrometer: performance characterization and use in the comparative analysis of histone H3 post-translational modifications J. Proteome Res. 3 3 621–6 2004 15253445 10.1021/pr0499794 or orbitrapMakarov A, Denisov E, Kholomeev A, Balschun W, Lange O, Strupat K, Horning S Performance evaluation of a hybrid linear ion trap/orbitrap mass spectrometer Anal. Chem. 78 7 2113–20 2006 16579588 10.1021/ac0518811Olsen JV, de Godoy LM, Li G, et al. Parts per million mass accuracy on an Orbitrap mass spectrometer via lock mass injection into a C-trap Mol. Cell Proteomics 4 12 2010–21 2005 December 16249172 10.1074/mcp.T500030-MCP200 http://www.mcponline.org/cgi/pmidlookup?view=long&pmid=16249172Yates JR, Cociorva D, Liao L, Zabrouskov V Performance of a linear ion trap-Orbitrap hybrid for peptide analysis Anal. Chem. 78 2 493–500 2006 January 16408932 10.1021/ac0514624 mass spectrometer is marketed by Thermo Scientific as the LTQ FT and LTQ Orbitrap, respectively. References