Chemical Sciences: A Manual for CSIR-UGC National Eligibility Test for Lectureship and JRF/Shotgun proteomics

Shotgun proteomics is a method of identifying proteins in complex mixtures using a combination of high performance liquid chromatography combined with mass spectrometry.Washburn MP, Wolters D, Yates JR Large-scale analysis of the yeast proteome by multidimensional protein identification technology Nat. Biotechnol. 19 3 242–247 2001 11231557 10.1038/85686 Wolters DA, Washburn MP, Yates JR An automated multidimensional protein identification technology for shotgun proteomics Anal. Chem. 73 23 5683–5690 2001 11774908 10.1021/ac010617e Hu L, Ye M, Jiang X, Feng S, Zou H Advances in hyphenated analytical techniques for shotgun proteome and peptidome analysis--a review Anal. Chim. Acta 598 2 193–204 2007 17719892 10.1016/j.aca.2007.07.046Fournier ML, Gilmore JM, Martin-Brown SA, Washburn MP Multidimensional separations-based shotgun proteomics Chem. Rev. 107 8 3654–86 2007 17649983 10.1021/cr068279aNesvizhskii AI Protein identification by tandem mass spectrometry and sequence database searching Methods Mol. Biol. 367 87–119 2007 17185772 10.1385/1-59745-275-0:87 The name is derived from shotgun sequencing of DNA which is itself named by analogy with the rapidly-expanding, quasi-random firing pattern of a shotgun. In shotgun proteomics, the proteins in the mixture are digested and the resulting peptides are separated by liquid chromatography. Tandem mass spectrometry is then used to identify the peptides. References Bibliography Marcotte EM How do shotgun proteomics algorithms identify proteins? Nat. Biotechnol. 25 7 755–7 2007 17621303 10.1038/nbt0707-755 http://www.nature.com/nbt/journal/v25/n7/full/nbt0707-755.html Veenstra, Timothy D.; Yates, John R. Proteomics for Biological Discovery Wiley-Liss New York 2006 0-471-16005-9