Chemical Sciences: A Manual for CSIR-UGC National Eligibility Test for Lectureship and JRF/Mass spectrometry imaging

Mass spectrometry imaging (also known as imaging mass spectrometry) is a technique used in mass spectrometry to visualize the spatial distribution of e.g. compounds, biomarker, metabolites, peptides or proteins by their molecular masses. Emerging technologies in the field of MSI are MALDI imaging and secondary ion mass spectrometry imaging (SIMS imaging) Rohner T, Staab D, Stoeckli M MALDI mass spectrometric imaging of biological tissue sections Mechanisms of Ageing and Development 126 1 177–185 2005 15610777 10.1016/j.mad.2004.09.032McDonnell LA, Heeren RM Imaging mass spectrometry Mass spectrometry reviews 26 4 606–43 2007 17471576 10.1002/mas.20124. SIMS Imaging Secondary ion mass spectrometry (SIMS) is used to analyze solid surfaces and thin films by sputtering the surface with a focused primary ion beam and collecting and analyzing ejected secondary ions. SIMS imaging is performed in a manner similar to electron microscopy; the primary ion beam is rastered across the sample while secondary mass spectra are recorded. MALDI imaging Matrix-assisted laser desorption ionization can be used as a mass spectrometry imaging technique for relatively large molecules.McDonnell LA, Heeren RM Imaging mass spectrometry Mass spectrometry reviews 26 4 606–43 2007 17471576 10.1002/mas.20124 In this version of the technique the sample, typically a thin tissue section, is moved in two dimensions while the mass spectrum is recorded.Chaurand P, Norris JL, Cornett DS, Mobley JA, Caprioli RM New developments in profiling and imaging of proteins from tissue sections by MALDI mass spectrometry J. Proteome Res. 5 11 2889–900 2006 17081040 10.1021/pr060346u DESI imaging Other mass spectrometry ionization methods can also be used for MS imaging: for example desorption electrospray ionization Ifa DR, Wiseman JM, Song Q, Cooks RG Development of capabilities for imaging mass spectrometry under ambient conditions with desorption electrospray ionization (DESI) International Journal of Mass Spectrometry 259 1-3 8–15 2007 10.1016/j.ijms.2006.08.003. References