Proteomics/Protein Separations- Electrophoresis/Timelines of Electrophoresis

__FORCETOC__ Gel Electrophoresis One Dimensional Gel Electrophoresis Two Dimensional Polyacrylamide Gel Electrophoresis(2D-PAGE) Differential in Gel Electrophoresis(DIGE) QPNC-PAGE Capillary Electrophoresis Timeline of Electrophoresis Databases Web Pages Online Applications Further Readings Timeline of Electrophoresis 1930s: Use of sucrose for gel electrophoresis. 1937: The official launching of Tiselius' moving boundary electrophoresis (MBE). 1939: Zone Electrophoresis 1950: Rich in introducing novel methodologies in zone electrophoresis (ZE) 1950: Agar Gel electrophoresis 1955: Introduction of starch gels 1957: Cellulose acetate Electrophoresis (CAc) by Kohn 1959: Introduction of acrylamide gels by Raymond and Winstraub 1961: Electrophoresis in silica gel by Honnegar 1961: IsoElectricFocusing by Svensson 1964: Electrophoresis in thin layer of sephadex beads by Fasella et.al 1964: Disc gel electrophoresis Ornstein and Davis 1969: Introduction of denaturing agents especially SDS separation of protein subunit by Beber and Osborn 1970: T4 phage proteins were separated using stacking gel and SDS by Laemmli 1975: 2 Dimensional gels: Isoelectric focusing and SDS gel electrophoresis by OFarrell. 1977: Sequencing gels 1970s: agars gels 1980s: Immobilized pH gradients 1983: Pulsed field gel electrophoresis 1983: Capillary electrophoresis References Electrophoresis: the march of pennies, the march of dimes. Righetti PG. http://www.csupomona.edu/~nebuckley/Courses/Bio451/Lectures/Bio451Lecture4.htm