GENtle/Silent Mutagenesis

thumb|The silent mutation dialog This dialog can find restriction enzymes that cut in a marked DNA sequence (context menu Selection/Show enzymes that cut here in the DNA module). It can also find alternate versions of the DNA which will translate into the same amino acid sequence, but contains a new restriction site (silent mutation). A chosen enzyme/mutation will appear in the sequence (DNA or primer, respectively) upon OK. The results can be changed by changing which enzyme group to search limiting the number of times an enzyme may cut in the whole sequence limiting the number of mutations needed for a restriction site to manifest (PCR module only)