2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

{{DISPLAYTITLE:2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase}} Class of enzymes 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase 4.6.1.12 0008685 2-C-Methyl-D-erythritol 2,4-cyclodiphosphate synthase (MEcPP synthase, IspF, EC 4.6.1.12) is a zinc-dependent enzyme and a member of the YgbB N terminal protein domain, which participates in the non-mevalonate pathway (MEP pathway) of isoprenoid precursor biosynthesis. It catalyzes the following reaction: Biosynthesis of terpenoids: YgbB protein converts 4-diphosphocytidyl-2C-methyl-d-erythritol 2-phosphate to 2C-methyl-d-erythritol 2,4-cyclodiphosphate Herz S, Wungsintaweekul J, Schuhr CA, Hecht S, Lüttgen H, Sagner S, Fellermeier M, Eisenreich W, Zenk MH, Bacher A, Rohdich F 2000 Proc. Natl. Acad. Sci. USA 6 10.1073/pnas.040554697 10694574 15955 97 2486–90 2000PNAS...97.2486H free 70% 4-Diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate CDP-MEP 2 Q27103069 MEcPP + cytidine monophosphate The reaction splits the precursor, 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-MEP) into 2-C-methyl-D-erythritol 2,4-cyclic diphosphate (MEcPP) and cytidine monophosphate. Most gram-negative bacteria, the photosynthetic cyanobacteria and green algae use only this pathway, while higher plants also use the mevalonate pathway.Rohmer M The discovery of a mevalonate-independent pathway for isoprenoid biosynthesis in bacteria, algae and higher plants Nat Prod Rep 16 5 565–574 1999 10584331 10.1039/a709175c Rohmer MichelVranová Eva Coman Diana Gruissem Wilhelm 2013-04-29 Network Analysis of the MVA and MEP Pathways for Isoprenoid Synthesis https://www.annualreviews.org/doi/10.1146/annurev-arplant-050312-120116 Annual Review of Plant Biology en 64 1 665–700 10.1146/annurev-arplant-050312-120116 23451776 2013AnRPB..64..665V 1543-5008 subscription The enzyme is a phosphorus-oxygen lyase. The systematic name of this enzyme class is 2-phospho-4-(cytidine 5′-diphospho)-2-C-methyl-D-D-erythritol CMP-lyase (cyclizing; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate-forming). Other names in common use include IspF, YgbB and MEcPP synthase.4.6.1.12 Structural studies As of late 2007, 20 structures have been solved for this class of enzymes, with PDB accession codes 1GX1, 1H47, 1H48, 1IV1, 1IV2, 1IV3, 1IV4, 1T0A, 1U3L, 1U3P, 1U40, 1U43, 1VH8, 1VHA, 1W55, 1W57, 1YQN, 2AMT, 2GZL, and 2PMP. References Biology no Category:EC 4.6.1 Category:Enzymes of known structure