2,4 Dienoyl-CoA reductase

Class of enzymes 2,4-dienoyl CoA reductase 1, mitochondrial DECR1, homotetramer, Human 1w6u.jpg 2753 DECR1 DECR 1666 222745 NM_001359 Q16698 1w6u 1.3.1.34 8 q 21.3 2,4 Dienoyl-CoA reductase also known as DECR1 is an enzyme which in humans is encoded by the DECR1 gene which resides on chromosome 8. This enzyme catalyzes the following reactionsEntrez Gene: 2,4-dienoyl CoA reductase 1, mitochondrial https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=ShowDetailView&TermToSearch=1666Koivuranta KT, Hakkola EH, Hiltunen JK Isolation and characterization of cDNA for human 120 kDa mitochondrial 2,4-dienoyl-coenzyme A reductase The Biochemical Journal 304 3 787–792 December 1994 7818482 1137403 10.1042/bj3040787 Helander HM, Koivuranta KT, Horelli-Kuitunen N, Palvimo JJ, Palotie A, Hiltunen JK Molecular cloning and characterization of the human mitochondrial 2,4-dienoyl-CoA reductase gene (DECR) Genomics 46 1 112–119 November 1997 9403065 10.1006/geno.1997.5004 File:Dienoyl-CoA reductase reaction cis-trans.svg DECR1 participates in the beta oxidation and metabolism of polyunsaturated fatty enoyl-CoA esters. Specifically, it catalyzes the reduction of 2,4 dienoyl-CoA thioesters of varying length by NADPH cofactor to 3-trans-enoyl-CoA of equivalent length. Unlike the breakdown of saturated fat, cis and trans polyunsaturated fatty acid degradation requires three additional enzymes to generate a product compatible with the standard beta oxidation pathway. DECR is the second such enzyme (the others being enoyl CoA isomerase and dienoyl CoA isomerase) and is the rate limiting step in this auxiliary flow. DECR is capable of reducing both 2-trans,4-cis-dienoyl-CoA and 2-trans,4-trans-dienoyl-CoA thioestersCuebas D, Schulz H Evidence for a modified pathway of linoleate degradation. Metabolism of 2,4-decadienoyl coenzyme A The Journal of Biological Chemistry 257 23 14140–14144 December 1982 7142199 10.1016/S0021-9258(19)45356-8 free with equal efficiency.Liang X, Thorpe C, Schulz H 2,4-Dienoyl-CoA reductase from Escherichia coli is a novel iron-sulfur flavoprotein that functions in fatty acid beta-oxidation Archives of Biochemistry and Biophysics 380 2 373–379 August 2000 10933894 10.1006/abbi.2000.1941 This is unusual, since most enzymes are highly stereoselective or stereospecific.Hanson Kenneth R. Rose Irwin A. 1975-01-01 Interpretations of enzyme reaction stereospecificity https://pubs.acs.org/doi/abs/10.1021/ar50085a001 Accounts of Chemical Research en 8 1 1–10 10.1021/ar50085a001 0001-4842 subscription There is no clear explanation for DECR's of lack of stereospecificity. Structure thumb|Crystallization1w6u; Alphey MS, Yu W, Byres E, Li D, Hunter WN Structure and reactivity of human mitochondrial 2,4-dienoyl-CoA reductase: enzyme-ligand interactions in a distinctive short-chain reductase active site The Journal of Biological Chemistry 280 4 3068–3077 January 2005 15531764 10.1074/jbc.M411069200 free of DECR with 2,4 Hexadienoyl-CoA and NADPH (not shown). Key residues in the enzyme active site orient the substrate for hydride transfer through a network of hydrogen bonds. Eukaryotic DECR exists in both the mitochondria (mDECR) and the peroxisome (pDECR, coded by gene DECR2). The enzymes from each organelle are homologous and part of the short-chain dehydrogenase/reductase SDR super-family. mDECR is 124 kDa consisting of 335 amino acids before post-translational modification. The secondary structure shares many of the motifs of SDR, including a Rossmann fold for strong NADPH binding. The protein exists as a homotetramer in physiological environment, but has been shown to also form monomers and dimers in solution.Yu W, Chu X, Chen G, Li D Studies of human mitochondrial 2,4-dienoyl-CoA reductase Archives of Biochemistry and Biophysics 434 1 195–200 February 2005 15629123 10.1016/j.abb.2004.10.018 Crystallization of mDECR shows the enzyme provides a network of hydrogen bonds from key residues in the active site to NADPH and the 2,4-dienoyl-CoA which positions the hydride at 3.4 Å to the Cδ, compared with 4.0 Å to the Cβ (not shown). The enolate intermediate discussed earlier is stabilized by residues additional hydrogen bonds to Tyr166 and Asn148. Lys214 and Ser210 (conserved residues in all SDR enzymes) are thought to increase the pKa of Tyr166 and stabilize the transition state. Additionally, at one end of the active site there is a flexible loop that provides sufficient room for long carbon chains. This likely gives the enzyme flexibility to process fatty acid chains of various lengths. Substrate length for mDECR catalysis is thought to be limited at 20 carbons, at which this very long chain fatty acid is first partially oxidized by pDECR in the peroxisome.Hua T, Wu D, Ding W, Wang J, Shaw N, Liu ZJ Studies of human 2,4-dienoyl CoA reductase shed new light on peroxisomal β-oxidation of unsaturated fatty acids The Journal of Biological Chemistry 287 34 28956–28965 August 2012 22745130 3436514 10.1074/jbc.M112.385351 free Enzyme mechanism Eukaryotic DECR 2,4 Dienoyl-CoA thioester reduction by NADPH to 3-Enoyl CoA occurs by a two-step sequential mechanism via an enolate intermediate.Fillgrove KL, Anderson VE The mechanism of dienoyl-CoA reduction by 2,4-dienoyl-CoA reductase is stepwise: observation of a dienolate intermediate Biochemistry 40 41 12412–12421 October 2001 11591162 10.1021/bi0111606 DECR binds NADPH and the fatty acid thioester and positions them for specific hydride transfer to the Cδ on the hydrocarbon chain. The electrons from the Cγ-Cδ double bond move over to the Cβ-Cγ position, and those from the Cα-Cβ form an enolate. In the final step, a proton is abstracted from the waterMizugaki M, Kimura C, Nishimaki T, Kawaguchi A, Okuda S, Yamanaka H Studies on the metabolism of unsaturated fatty acids. XII. Reaction catalyzed by 2,4-dienoyl-CoA reductase of Escherichia coli Journal of Biochemistry 94 2 409–413 August 1983 6355075 10.1093/oxfordjournals.jbchem.a134370 free to the Cα and the thioester is reformed, resulting in a single Cβ-Cγ trans double bond. Since the final proton comes from water, the pH has a significant effect on the catalytic rate with the enzyme demonstrating maximal activity at ~6.0. A decrease in activity at pH < 6.0 can be explained by de-protonation of titratable residues that affect protein folding or substrate binding. Mutant proteins with modifications at key acidic amino acids (E154, E227, E276, D300, D117) show order of magnitude increases in Km and/or decreases in Vmax. thumb|Proposed mechanism of 2,4-Trans dienoyl-CoA reduction by NADPH in mammalian DECR. The mechanism proceeds stepwise through an enolate intermediate. Prokaryotic DECR 2,4 Dienoyl-CoA Reductase from Escherichia coli shares very similar kinetic properties to that of eukaryotes, but differs significantly in both structure and mechanism. In addition to NADPH, E. Coli DECR requires a set of FAD, FMN and iron–sulfur cluster molecules to complete the electron transfer.Liang X, Thorpe C, Schulz H 2,4-Dienoyl-CoA reductase from Escherichia coli is a novel iron-sulfur flavoprotein that functions in fatty acid beta-oxidation Archives of Biochemistry and Biophysics 380 2 373–379 August 2000 10933894 10.1006/abbi.2000.1941 A further distinction is E. Coli DECR produces the final 2-trans-enoyl-CoA without the need for Enoyl CoA Isomerase. The active site contains accurately positioned Tyr166 that donates a proton to the Cγ after hydride attack at the Cδ, completing the reduction in a single concerted step.Hubbard PA, Liang X, Schulz H, Kim JJ The crystal structure and reaction mechanism of Escherichia coli 2,4-dienoyl-CoA reductase The Journal of Biological Chemistry 278 39 37553–37560 September 2003 12840019 10.1074/jbc.M304642200 free Surprisingly, mutation of the Tyr166 does not eliminate enzyme activity but instead changes the product to 3-trans-enoyl-CoA. The current explanation is that Glu164, an acidic residue in the active site, acts as a proton donor to Cα when Tyr166 is not present.Tu X, Hubbard PA, Kim JJ, Schulz H Two distinct proton donors at the active site of Escherichia coli 2,4-dienoyl-CoA reductase are responsible for the formation of different products Biochemistry 47 4 1167–1175 January 2008 18171025 10.1021/bi701235t Function DECR is one of three auxiliary enzymes involved in a rate-limiting step of unsaturated fatty acid oxidation in mitochondria. In particular, this enzyme contributes to breaking the double bonds at all even-numbered positions, and some double bonds at odd-numbered position. The structure of the ternary complex of pDCR (peroxisomal 2,4-dienoyl CoA reductases) with NADP and its substrate provides essential and unique insights into the mechanism of catalysis.Ylianttila MS, Pursiainen NV, Haapalainen AM, Juffer AH, Poirier Y, Hiltunen JK, Glumoff T Crystal structure of yeast peroxisomal multifunctional enzyme: structural basis for substrate specificity of (3R)-hydroxyacyl-CoA dehydrogenase units Journal of Molecular Biology 358 5 1286–1295 May 2006 16574148 10.1016/j.jmb.2006.03.001 Unlike other members belonging to the SDR family, catalysis by pDCR does not involve a tyrosine-serine pair. Instead, a catalytically critical aspartate, together with an invariant lysine, polarizes a water molecule to donate a proton for the formation of the product. Although pDCR can use 2,4-hexadienoyl CoA as a substrate, the affinities for short chain fatty acids are lower. Analysis of the hinge movement of DCRs from the mitochondrion and peroxisomes sheds light on the reason behind the unique ability of the peroxisome to shorten very long chain fatty acids.Emekli U, Schneidman-Duhovny D, Wolfson HJ, Nussinov R, Haliloglu T HingeProt: automated prediction of hinges in protein structures Proteins 70 4 1219–1227 March 2008 17847101 10.1002/prot.21613 free 26975077 Clinical significance Mutations in the DECR1 gene may result in 2,4 Dienoyl-CoA reductase deficiency,Roe CR, Millington DS, Norwood DL, Kodo N, Sprecher H, Mohammed BS, Nada M, Schulz H, McVie R 2,4-Dienoyl-coenzyme A reductase deficiency: a possible new disorder of fatty acid oxidation The Journal of Clinical Investigation 85 5 1703–1707 May 1990 2332510 296625 10.1172/JCI114624 6 a rare but lethal disorder. Due to its role in fatty acid oxidation, DECR may serve as a therapeutic target for treating non-insulin dependent diabetes mellitus (NIDDM), which features hyperglycemia due to increased fatty acid oxidation. In knockout mice studies, DECR1−/− subjects accumulate significant concentrations of mono and polyunsaturated fatty acids in the liver during fasting (such as oleic acid, palmitoleic acid, linoleic acid, and linolenic acid). Mutant subjects were also found to have poor tolerance to cold, decrease in diurnal activity, and an overall reduction in adaptation to metabolic stressors.Miinalainen IJ, Schmitz W, Huotari A, Autio KJ, Soininen R, Ver Loren van Themaat E, Baes M, Herzig KH, Conzelmann E, Hiltunen JK Mitochondrial 2,4-dienoyl-CoA reductase deficiency in mice results in severe hypoglycemia with stress intolerance and unimpaired ketogenesis PLOS Genetics 5 7 July 2009 19578400 2697383 10.1371/journal.pgen.1000543 free 6 e1000543 See also Beta oxidation 2,4-dienoyl-CoA reductase 1 References External links 2,4-dienoyl-CoA+reductase Biology no Category:EC 1.3.1