2,7,4'-Trihydroxyisoflavanone 4'-O-methyltransferase

Class of enzymes 2,7,4'-Trihydroxyisoflavanone 4'-O-methyltransferase 2.1.1.212 2,7,4'-Trihydroxyisoflavanone 4'-O-methyltransferase (2.1.1.212, SAM:2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase, HI4'OMT, HMM1, MtIOMT5) is an enzyme with systematic name S-adenosyl-L-methionine:2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase. Akashi T, Sawada Y, Shimada N, Sakurai N, Aoki T, Ayabe S cDNA cloning and biochemical characterization of S-adenosyl-L-methionine: 2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase, a critical enzyme of the legume isoflavonoid phytoalexin pathway Plant & Cell Physiology 44 2 103–12 February 2003 12610212 10.1093/pcp/pcg034 free Liu CJ, Deavours BE, Richard SB, Ferrer JL, Blount JW, Huhman D, Dixon RA, Noel JP Structural basis for dual functionality of isoflavonoid O-methyltransferases in the evolution of plant defense responses The Plant Cell 18 12 3656–69 December 2006 17172354 1785397 10.1105/tpc.106.041376 2006PlanC..18.3656L Akashi T, VanEtten HD, Sawada Y, Wasmann CC, Uchiyama H, Ayabe S Catalytic specificity of pea O-methyltransferases suggests gene duplication for (+)-pisatin biosynthesis Phytochemistry 67 23 2525–30 December 2006 10.1016/j.phytochem.2006.09.010 17067644 2006PChem..67.2525A This enzyme catalyses the following chemical reaction 65% Q27123131 2,4',7-trihydroxyisoflavanone + SAM Q27132128 2,7-dihydroxy-4'-methoxyisoflavanone + SAH This is a methylation reaction in which the isoflavanone, 2,4',7-trihydroxyisoflavanone, is converted a specific methyl ether, 2,7-dihydroxy-4'-methoxyisoflavanone. The methyl group comes from the cofactor, S-adenosyl methionine (SAM), which becomes S-adenosyl-L-homocysteine (SAH). The enzyme was characterised from the legume, Medicago truncatula. Deavours BE, Liu CJ, Naoumkina MA, Tang Y, Farag MA, Sumner LW, Noel JP, Dixon RA Functional analysis of members of the isoflavone and isoflavanone O-methyltransferase enzyme families from the model legume Medicago truncatula Plant Molecular Biology 62 4–5 715–33 November 2006 17001495 2862459 10.1007/s11103-006-9050-x 2006PMolB..62..715D References External links 2,7,4'-trihydroxyisoflavanone+4'-O-methyltransferase Biology no Category:EC 2.1.1