2-Carboxy-D-arabinitol-1-phosphatase

Class of enzymes 2-Carboxy-D-arabinitol-1-phosphatase 3.1.3.63 122319-88-4 0047538 The enzyme 2-carboxy-D-arabinitol-1-phosphatase (CA1Pase; EC 3.1.3.63) catalyzes the reaction 2-carboxy-D-arabinitol 1-phosphate + H2O 2-carboxy-D-arabinitol + phosphate This enzyme belongs to the family of hydrolases, to be specific, those acting on phosphoric monoester bonds. The systematic name is 2-carboxy-D-arabinitol-1-phosphate 1-phosphohydrolase. In biology The best-studied 2-Carboxy-D-arabinitol-1-phosphate phosphatase is the enzyme that inactivates the RuBisCO inhibitor 2-carboxy-D-arabinitol-1-phosphate (CA1P). When light levels are high, the inactivation occurs after CA1P has been released from RuBisCO by RuBisCO activase. As CA1P is present in many but not all plants, CA1P-mediated regulation of RuBisCO is not universal for all photosynthetic life. Amino acid sequences of the CA1Pase enzymes from wheat, French bean, tobacco, and Arabidopsis thaliana reveal that the enzymes contain 2 different domains, indicating that it is a multifunctional enzyme. CA1Pase enzyme activity varies between different species due to their regulation by different redox-active compounds, such as glutathione. However, it is yet to be determined whether this process occurs in vivo. Wheat CA1Pase heterologously expressed in E. coli is also able to dephosphorylate the RuBisCO inhibitor D-glycero-2,3-diulose-1,5-bisphosphate. Andralojc PJ, Madgwick PJ, Tao Y, Keys A, Ward JL, Beale MH, Loveland JE, Jackson PJ, Willis AC, Gutteridge S, Parry MA 6 2-Carboxy-D-arabinitol 1-phosphate (CA1P) phosphatase: evidence for a wider role in plant Rubisco regulation The Biochemical Journal 442 3 733–42 March 2012 22132794 10.1042/BJ20111443 References 1 Further reading Salvucci ME, Holbrook GP Purification and Properties of 2-Carboxy-D-Arabinitol 1-Phosphatase Plant Physiology 90 2 679–85 June 1989 16666827 1061780 10.1104/pp.90.2.679 Biology no Category:EC 3.1.3 Category:Enzymes of unknown structure