2'-phosphotransferase

Class of enzymes 2'-phosphotransferase 2.7.1.160 In enzymology, a 2'-phosphotransferase (2.7.1.160) is an enzyme that catalyzes the chemical reaction 2'-phospho-[ligated tRNA] + NAD+ mature tRNA + ADP-ribose 1,2-phosphate + nicotinamide + H2O Thus, the two substrates of this enzyme are [[2'-phospho-[ligated tRNA]]] and NAD+, whereas its 4 products are mature tRNA, ADP-ribose 1,2-phosphate, nicotinamide, and H2O. This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. The systematic name of this enzyme class is 2'-phospho-[ligated tRNA]:NAD+ phosphotransferase. Other names in common use include yeast 2'-phosphotransferase, Tpt1, Tpt1p, and 2'-phospho-tRNA:NAD+ phosphotransferase. References 1 Steiger MA, Kierzek R, Turner DH, Phizicky EM 2001 Substrate recognition by a yeast 2'-phosphotransferase involved in tRNA splicing and by its Escherichia coli homolog Biochemistry 40 14098–105 11705403 10.1021/bi011388t 46 Spinelli SL, Kierzek R, Turner DH, Phizicky EM 1999 Transient ADP-ribosylation of a 2'-phosphate implicated in its removal from ligated tRNA during splicing in yeast J. Biol. Chem. 274 2637–44 9915792 10.1074/jbc.274.5.2637 5 free Culver GM, McCraith SM, Consaul SA, Stanford DR, Phizicky EM 1997 A 2'-phosphotransferase implicated in tRNA splicing is essential in Saccharomyces cerevisiae J. Biol. Chem. 272 13203–10 9148937 10.1074/jbc.272.20.13203 20 free McCraith SM, Phizicky EM 1991 An enzyme from Saccharomyces cerevisiae uses NAD+ to transfer the splice junction 2'-phosphate from ligated tRNA to an acceptor molecule J. Biol. Chem. 266 11986–92 2050693 18 10.1016/S0021-9258(18)99054-X free Hu QD, Lu H, Huo K, Ying K, Li J, Xie Y, Mao Y, Li YY 2003 A human homolog of the yeast gene encoding tRNA 2'-phosphotransferase: cloning, characterization and complementation analysis Cell. Mol. Life Sci. 60 1725–32 14504659 10.1007/s00018-003-3107-7 8 11138648 Steiger MA, Jackman JE, Phizicky EM 2005 Analysis of 2'-phosphotransferase (Tpt1p) from Saccharomyces cerevisiae: evidence for a conserved two-step reaction mechanism RNA 11 99–106 15611300 10.1261/rna.7194605 1 1370695 Sawaya R, Schwer B, Shuman S 2005 Structure-function analysis of the yeast NAD+-dependent tRNA 2'-phosphotransferase Tpt1 RNA 11 107–13 15611301 10.1261/rna.7193705 1 1370696 Kato-Murayama M, Bessho Y, Shirouzu M, Yokoyama S 2005 Crystal structure of the RNA 2'-phosphotransferase from Aeropyrum pernix K1 J. Mol. Biol. 348 295–305 15811369 10.1016/j.jmb.2005.02.049 2 Biology no Category:EC 2.7.1 Category:NADH-dependent enzymes Category:Enzymes of unknown structure