(R)-amidase

Class of enzymes (R)-amidase 3.5.1.100 (R)-amidase (3.5.1.100, R-stereospecific amidase, R-amidase) is an enzyme with systematic name (R)-piperazine-2-carboxamide amidohydrolase.3.5.1.100 The enzyme characterised from Pseudomonas species catalyses several related hydrolysis reactions. Komeda H, Harada H, Washika S, Sakamoto T, Ueda M, Asano Y A novel R-stereoselective amidase from Pseudomonas sp. MCI3434 acting on piperazine-2-tert-butylcarboxamide European Journal of Biochemistry 271 8 1580–90 April 2004 15066183 10.1111/j.1432-1033.2004.04069.x For example, it converts β-alaninamide to β-alanine: 50% Q27124258 β-alaninamide H2O NH3 β-Alanine β-alanine It also acts on (R)-piperazine-2-carboxamide: 50% Q27124252 (R)-piperazine-2-carboxamide H2O NH3 Q27124253 (R)-piperazine-2-carboxylic acid Other substrates include (R)-N-tert-butylpiperazine-2-carboxamide and (R)-piperidine-3-carboxamide. Similar amidases from other organisms are known,Ruan Li-Tao Zheng Ren-Chao Zheng Yu-Guo Shen Yin-Chu Purification and characterization of R -stereospecific amidase from Brevibacterium epidermidis ZJB-07021 International Journal of Biological Macromolecules 2016 86 893–900 10.1016/j.ijbiomac.2016.02.020 26868191 including one dependent on cobalt for activity.Wu Zhe-Ming Zheng Ren-Chao Tang Xiao-Ling Zheng Yu-Guo Identification and characterization of a thermostable and cobalt-dependent amidase from Burkholderia phytofirmans ZJB-15079 for efficient synthesis of (R)-3,3,3-trifluoro-2-hydroxy-2-methylpropionic acid Applied Microbiology and Biotechnology 2017 101 5 1953–1964 10.1007/s00253-016-7921-x 27832306 Immobilized enzyme has been used to produce single-enantiomer piperazine-2-carboxylic acid from the racemic amide.Roura Padrosa David Wetzl Dennis Hildbrand Stefan Tosatti Paolo Sedelmeier Joerg Puentener Kurt Iding Hans Paradisi Francesca Biocatalyst Immobilization and Process Intensification: Production of ( S )-Piperazine-2-carboxylic Acid Dihydrochloride Organic Process Research & Development 2024 28 5 1713–1724 10.1021/acs.oprd.3c00347 References External links (R)-amidase Biology no Category:EC 3.5.1