2-oxopropyl-CoM reductase (carboxylating)

Class of enzymes 2-oxopropyl-CoM reductase (carboxylating) 1.8.1.5 0050628 2-oxopropyl-CoM reductase (carboxylating) (1.8.1.5) is an enzyme that catalyzes the chemical reaction 70% Q27102715 Coenzyme M + NADP+ + acetoacetic acid reversible CO2 + H+ CO2 + H+ Q72435869 2-acetylthioethanesulfonic acid 1.2 + NADPH The three substrates of this enzyme are 2-mercaptoethanesulfonic acid (Coenzyme M), acetoacetic acid, and nicotinamide adenine dinucleotide phosphate (NADP+). Its products are 2-acetylthioethanesulfonic acid (2-oxopropyl-Coenzyme M), carbon dioxide, reduced NADPH, and a proton.1.8.1.5Allen JR, Clark DD, Krum JG, Ensign SA 1999 A role for coenzyme M (2-mercaptoethanesulfonic acid) in a bacterial pathway of aliphatic epoxide carboxylation Proc. Natl. Acad. Sci. U.S.A. 96 8432–7 10411892 10.1073/pnas.96.15.8432 15 17533 1999PNAS...96.8432A free Clark DD, Allen JR, Ensign SA 2000 Characterization of five catalytic activities associated with the NADPH:2-ketopropyl-coenzyme M [2-(2-ketopropylthio)ethanesulfonate] oxidoreductase/carboxylase of the Xanthobacter strain Py2 epoxide carboxylase system Biochemistry 39 1294–304 10684609 10.1021/bi992282p 6 This enzyme belongs to the family of oxidoreductases, specifically those acting on a sulfur group of donors with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is 2-mercaptoethanesulfonate, acetoacetate:NADP+ oxidoreductase (decarboxylating). Other names in common use include NADPH:2-(2-ketopropylthio)ethanesulfonate, oxidoreductase/carboxylase, and NADPH:2-ketopropyl-coenzyme M oxidoreductase/carboxylase. Structural studies As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 2C3C and 2C3D. References Biology no Category:EC 1.8.1 Category:NADPH-dependent enzymes Category:Enzymes of known structure