(Formate-C-acetyltransferase)-activating enzyme

Class of enzymes [formate-C-acetyltransferase]-activating enzyme 1.97.1.4 206367-15-9 0043365 In enzymology, a [formate-C-acetyltransferase]-activating enzyme (1.97.1.4) is an enzyme that catalyzes the chemical reaction S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical The 3 substrates of this enzyme are S-adenosyl-L-methionine, dihydroflavodoxin, and formate C-acetyltransferase-glycine, whereas its 4 products are 5'-deoxyadenosine, L-methionine, flavodoxin semiquinone, and formate C-acetyltransferase-glycin-2-yl radical. This radical SAM enzyme belongs to the family of oxidoreductases. The systematic name of this enzyme class is [formate C-acetyltransferase]-glycine dihydroflavodoxin:S-adenosyl-L-methionine oxidoreductase (S-adenosyl-L-methionine cleaving). Other names in common use include PFL activase, PFL-glycine:S-adenosyl-L-methionine H transferase (flavodoxin-oxidizing, S-adenosyl-L-methionine-cleaving), formate acetyltransferase activating enzyme, formate acetyltransferase-glycine dihydroflavodoxin:S-adenosyl-L-methionine oxidoreductase (S-adenosyl-L-methionine cleaving). References 1 Frey M, Rothe M, Wagner AF, Knappe J 1994 Adenosylmethionine-dependent synthesis of the glycyl radical in pyruvate formate-lyase by abstraction of the glycine C-2 pro-S hydrogen atom. Studies of [2H]glycine-substituted enzyme and peptides homologous to the glycine 734 site J. Biol. Chem. 269 12432–7 8175649 17 10.1016/S0021-9258(18)99892-3 free Wagner AF, Frey M, Neugebauer FA, Schafer W, Knappe J 1992 The free radical in pyruvate formate-lyase is located on glycine-734 Proc. Natl. Acad. Sci. U.S.A. 89 996–1000 1310545 10.1073/pnas.89.3.996 3 48372 1992PNAS...89..996W free Frey PA 2001 Radical mechanisms of enzymatic catalysis Annu. Rev. Biochem. 70 121–48 11395404 10.1146/annurev.biochem.70.1.121 Biology no Category:EC 1.97.1 Category:Enzymes of unknown structure