1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming)

Enzyme in the family of oxidoreductases 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) 1.1.1.292 In enzymology, 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) (1.1.1.292) is an enzyme that catalyzes the chemical reaction 50% Q27121518 1,5-anhydro-D-mannitol + NADP+ reversible H+ H+ Q27102040 1,5-anhydro-D-fructose + NADPH The two substrates of this enzyme are 1,5-anhydro-D-mannitol and oxidised nicotinamide adenine dinucleotide phosphate (NADP+). Its products are 1,5-anhydro-D-fructose, reduced NADPH, and a proton.1.1.1.292Kuhn A, Yu S, Giffhorn F 2006 Catabolism of 1,5-anhydro-D-fructose in Sinorhizobium morelense S-30.7.5: discovery, characterization, and overexpression of a new 1,5-anhydro-D-fructose reductase and its application in sugar analysis and rare sugar synthesis Appl. Environ. Microbiol. 72 1248–57 16461673 10.1128/AEM.72.2.1248-1257.2006 2 1392929 2006ApEnM..72.1248K Dambe TR, Kuhn AM, Brossette T, Giffhorn F, Scheidig AJ 2006 Crystal structure of NADP(H)-dependent 1,5-anhydro-D-fructose reductase from Sinorhizobium morelense at 2.2 Å resolution: construction of an NADH-accepting mutant and its application in rare sugar synthesis Biochemistry 45 10030–42 16906761 10.1021/bi052589q 33 This enzyme belongs to the common family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is 1,5-anhydro-D-mannitol:NADP+ oxidoreductase. Other names in common use include 1,5-anhydro-D-fructose reductase (ambiguous), and AFR. References 1 Biology no Category:EC 1.1.1 Category:NADPH-dependent enzymes Category:Enzymes of unknown structure