2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Class of enzymes 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 2.7.7.60 251990-59-7 0050518 IspD IspD PDB 1w77 EBI.jpg 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (IspD) from Arabidopsis thaliana PF01128 CL0110 IPR001228 PDOC00997 1inj 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (2.7.7.60) is an enzyme that catalyzes the chemical reaction: 70% 2-C-Methylerythritol 4-phosphate 2-C-methylerythritol 4-phosphate + cytidine triphosphate 1.2 PPi 4-Diphosphocytidyl-2-C-methylerythritol CDP-ME 1.3 The enzyme has been characterised in Escherichia coli and Arabidopsis thaliana. The reaction is the third step of the MEP pathway to terpenes; the formation of CDP-ME (4-diphosphocytidyl-2C-methylerythritol) from cytidine triphosphate and 2C-methyl-D-erythritol 4-phosphate (MEP). Rohdich F, Wungsintaweekul J, Fellermeier M etal 1999 Cytidine 5'-triphosphate-dependent biosynthesis of isoprenoids: YgbP protein of Escherichia coli catalyzes the formation of 4-diphosphocytidyl-2-C-methylerythritol Proc. Natl. Acad. Sci. USA 96 11758–63 10518523 10.1073/pnas.96.21.11758 21 18359 free 1999PNAS...9611758R Rohdich F, Wungsintaweekul J, Eisenreich W, Richter G, Schuhr CA, Hecht S, Zenk MH, Bacher A Biosynthesis of terpenoids: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase of Arabidopsis thaliana Proceedings of the National Academy of Sciences of the United States of America 97 12 6451–6 June 2000 10841550 18623 10.1073/pnas.97.12.6451 2000PNAS...97.6451R free The isoprenoid pathway is a well known target for anti-infective drug development.Illarionova V, Kaiser J, Ostrozhenkova E, Bacher A, Fischer M, Eisenreich W, Rohdich F Nonmevalonate terpene biosynthesis enzymes as antiinfective drug targets: substrate synthesis and high-throughput screening methods J. Org. Chem. 71 23 8824–34 November 2006 17081012 10.1021/jo061466o Eoh H, Brown AC, Buetow L, Hunter WN, Parish T, Kaur D, Brennan PJ, Crick DC Characterization of the Mycobacterium tuberculosis 4-diphosphocytidyl-2-C-methyl-D-erythritol synthase: potential for drug development J. Bacteriol. 189 24 8922–7 December 2007 17921290 2168624 10.1128/JB.00925-07 Kuzuyama T Takagi M Kaneda K Dairi T Seto H 2000 Formation of 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol from 2-C-methyl-D-erythritol 4-phosphate by 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, a new enzyme in the nonmevalonate pathway Tetrahedron Lett. 41 50 703–706 10.1016/S0040-4039(99)02143-7 Nomenclature This enzyme is a transferase, specifically one transferring phosphorus-containing nucleotide groups (nucleotidyltransferases). Its systematic name is CTP:2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase and it is also called MEP cytidylyltransferase or CDP-ME synthetase.2.7.7.60 It is normally abbreviated IspD and referenced by the open reading frame YgbP. Structural studies The crystal structure of the Escherichia coli 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1I52, 1INI & 1INJ, reported by Richard et al. (2001),Richard SB, Bowman ME, Kwiatkowski W, Kang I, Chow C, Lillo AM, Cane DE, Noel JP 2001 Structure of 4-diphosphocytidyl-2-C- methylerythritol synthetase involved in mevalonate- independent isoprenoid biosynthesis. Nature Structural & Molecular Biology 8 641–8 11427897 10.1038/89691 7 8494543 was the first one for an enzyme involved in the MEP pathway. As of February 2010, 13 other structures have been solved for this class of enzymes, with PDB accession codes 1H3M, 1VGT, 1VGU, 1VGZ, 1VPA, 1VGW, 1W55, 1W57, 1W77,2PX7, 2VSI, 3F1C and 2VSH. References IPR001228 Biology no Category:EC 2.7.7 Category:Enzymes of known structure