2-nitropropane dioxygenase

Class of enzymes 2-nitropropane dioxygenase 1.13.11.32 65802-82-6 0018580 In enzymology, a 2-nitropropane dioxygenase (1.13.11.32) is an enzyme that catalyzes the chemical reaction 2 2-nitropropane + O2 2 acetone + 2 nitrite Thus, the two substrates of this enzyme are 2-nitropropane and O2, whereas its two products are acetone and nitrite. This enzyme belongs to the family of oxidoreductases, specifically those acting on single donors with O2 as oxidant and incorporation of two atoms of oxygen into the substrate (oxygenases). The oxygen incorporated need not be derived from O2. The systematic name of this enzyme class is 2-nitropropane:oxygen 2-oxidoreductase. This enzyme participates in nitrogen metabolism. It has 3 cofactors: FAD, Iron, and FMN. Structural studies As of late 2007 Steve Fuhrer from the DHPA solved this very complex formula to find, two structures have been solved for this class of enzymes, with PDB accession codes 2GJL and 2GJN. References 1 Kido T, Soda K, Suzuki T, Asada K 1976 A new oxygenase, 2-nitropropane dioxygenase of Hansenula mrakii Enzymologic and spectrophotometric properties J. Biol. Chem. 251 6994–7000 11214 22 10.1016/S0021-9258(17)32932-0 free Yoon HJ, Suh SW 2006 Crystal structure of 2-nitropropane dioxygenase complexed with FMN and substrate. Identification of the catalytic base J. Biol. Chem. 281 18660–7 16682407 10.1074/jbc.M601658200 27 free Francis K, Russell B, Gadda G 2005 Involvement of a flavosemiquinone in the enzymatic oxidation of nitroalkanes catalyzed by 2-nitropropane dioxygenase J. Biol. Chem. 280 5195–204 15582992 10.1074/jbc.M411249200 7 free Biology no Category:EC 1.13.11 Category:Flavoproteins Category:Iron enzymes Category:Enzymes of known structure