1,2-α-L-fucosidase

Class of enzymes 1,2-α-L-Fucosidase 3.2.1.63 37288-45-2 0047513 The enzyme 1,2-α-L-fucosidase (3.2.1.63) catalyzes the following chemical reaction: 70% Q27102435 (1) H2O Q409082 L-fucose + Q27094900 methyl β-D-galactoside The enzyme characterised from Aspergillus niger, Chamelea gallina, and almonds hydrolyses methyl-2-α-L-fucopyranosyl-β-D-galactoside (1) to L-fucose and methyl β-D-galactoside. Bahl OP 1970 Glycosidases of Aspergillus niger. II. Purification and general properties of 1,2-α-L-fucosidase J. Biol. Chem. 245 299–304 5460888 2 10.1016/S0021-9258(18)63394-0 free Reglero A, Cabezas JA 1976 Glycosidases of molluscs. Purification and properties of α-L-fucosidase from Chamelea gallina L Eur. J. Biochem. 66 379–87 7458 10.1111/j.1432-1033.1976.tb10527.x 2 free Ogata-Arakawa M, Muramatsu T, Kobata A 1977 α-L-Fucosidases from almond emulsin: characterization of the two enzymes with different specificities Arch. Biochem. Biophys. 181 353–8 18111 10.1016/0003-9861(77)90514-8 1 The mechanism of the reaction has been studied by X-ray crystallography of isolates from Bifidobacterium bifidum which are structurally similar to bacterial phosphorylases and shown to involve inversion at the anomeric centre.Nagae Masamichi Tsuchiya Atsuko Katayama Takane Yamamoto Kenji Wakatsuki Soichi Kato Ryuichi Structural Basis of the Catalytic Reaction Mechanism of Novel 1,2-α-L-Fucosidase from Bifidobacterium bifidum Journal of Biological Chemistry 2007 282 25 18497–18509 10.1074/jbc.M702246200 free 17459873 It has been suggested that the enzyme from that source be added to pig food in commercial production to reduce methane emissions by promoting beneficial gut bacteria and reduce methanogens.Georgaki D. Canibe N. Van Der Heide M. E. Foldager L. Poulsen C. H. Mejldal R. Velayudhan D. Nørgaard J. V. Højberg O. 3 Impact of 1,2-α-L-fucosidase on pig enteric methane production and growth performance Animal Microbiome 2026 8 1 30 10.1186/s42523-025-00487-9 free 41857672 13001275 It is a hydrolase, specifically a glycosidase that hydrolyses O- and S-glycosyl compounds. The systematic name is 2-α-L-fucopyranosyl-β-D-galactoside fucohydrolase. Other names in use include almond emulsin fucosidase, and α-(1→2)-L-fucosidase.3.2.1.63 Structural studies As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes 2EAB, 2EAC, 2EAD, and 2EAE. References Biology no Category:EC 3.2.1 Category:Enzymes of known structure