2,5-didehydrogluconate reductase

Class of enzymes 2,5-didehydrogluconate reductase 1.1.1.274 95725-95-4 0050580 In enzymology, 2,5-didehydrogluconate reductase (1.1.1.274) is an enzyme that catalyzes the chemical reaction 50% Q27103150 2-dehydro-D-gluconic acid + NADP+ reversible H+ H+ Q27102964 2,5-didehydro-D-gluconic acid + NADPH The two substrates of this enzyme are 2-dehydro-D-gluconic acid and oxidised nicotinamide adenine dinucleotide phosphate (NADP+). Its products are 2,5-didehydro-D-gluconic acid, reduced NADPH, and a proton.1.1.1.274Yum DY, Lee BY, Pan JG 1999 Identification of the yqhE and yafB genes encoding two 2, 5-diketo-D-gluconate reductases in Escherichia coli Appl. Environ. Microbiol. 65 3341–6 10427017 8 10.1128/AEM.65.8.3341-3346.1999 91502 1999ApEnM..65.3341Y Yum DY, Lee BY, Hahm DH, Pan JG 1998 The yiaE gene, located at 80.1 minutes on the Escherichia coli chromosome, encodes a 2-ketoaldonate reductase J. Bacteriol. 180 5984–8 9811658 22 10.1128/JB.180.22.5984-5988.1998 107674 Habrych M, Rodriguez S, Stewart JD 2002 Purification and identification of an Escherichia coli beta-keto ester reductase as 2,5-diketo-D-gluconate reductase YqhE Biotechnol. Prog. 18 257–61 11934293 10.1021/bp0101841 2 23141855 This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is 2-dehydro-D-gluconate:NADP+ 2-oxidoreductase. Other names in common use include 2,5-diketo-D-gluconate reductase, and YqhE reductase. Structural studies As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code 1VP5. References Biology no Category:EC 1.1.1 Category:NADPH-dependent enzymes Category:Enzymes of known structure