(S)-2-hydroxy-acid oxidase

Class of enzymes (S)-2-hydroxy-acid oxidase 1.1.3.15 9037-63-2 0003973 2rdw.jpg 270 Glycolate oxidase tetramer, Human In enzymology, an (S)-2-hydroxy-acid oxidase (1.1.3.15) is an enzyme that catalyzes the chemical reaction (S)-2-hydroxy acid + O2 2-oxo acid + H2O2 Thus, the two substrates of this enzyme are (S)-2-hydroxy acid and O2, whereas its two products are 2-oxo acid and H2O2. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. The systematic name of this enzyme class is (S)-2-hydroxy-acid:oxygen 2-oxidoreductase. Other names in common use include glycolate oxidase, hydroxy-acid oxidase A, hydroxy-acid oxidase B, oxidase, L-2-hydroxy acid, hydroxyacid oxidase A, L-alpha-hydroxy acid oxidase, and L-2-hydroxy acid oxidase. This enzyme participates in glyoxylate and dicarboxylate metabolism. It employs one cofactor, FMN. Structural studies As of late 2007, 5 structures have been solved for this class of enzymes, with PDB accession codes 1AL7, 1AL8, 1GYL, 1TB3, and 2NZL. References 1 Blanchard M, Green DE, Nocito-Carroll V, Ratner S 1946 l-Hydroxy acid oxidase J. Biol. Chem. 163 137–144 http://www.jbc.org/content/163/1/137.full.pdf 1 10.1016/S0021-9258(17)41353-6 21023634 free FRIGERIO NA, HARBURY HA 1958 Preparation and some properties of crystalline glycolic acid oxidase of spinach J. Biol. Chem. 231 135–57 13538955 1 10.1016/S0021-9258(19)77292-5 free KUN E, DECHARY JM, PITOT HC 1954 The oxidation of glycolic acid by a liver enzyme J. Biol. Chem. 210 269–80 13201588 1 10.1016/S0021-9258(18)65451-1 free Nakano M, Danowski TS 1966 Crystalline mammalian L-amino acid oxidase from rat kidney mitochondria J. Biol. Chem. 241 2075–83 5946631 9 10.1016/S0021-9258(18)96668-8 free Nakano M, Ushijima Y, Saga M, Tsutsumi Y, Asami H 1968 Aliphatic L-alpha-hydroxyacid oxidase from rat livers: purification and properties Biochim. Biophys. Acta 167 9–22 5686300 1 10.1016/0005-2744(68)90273-8 Schuman M, Massey V 1971 Purification and characterization of glycolic acid oxidase from pig liver Biochim. Biophys. Acta 227 500–20 5569122 3 10.1016/0005-2744(71)90003-9 2027.42/33685 https://deepblue.lib.umich.edu/bitstream/2027.42/33685/1/0000197.pdf free Phillips DR, Duley JA, Fennell DJ, Holmes RS 1976 The self-association of L-alpha hydroxyacid oxidase Biochim. Biophys. Acta 427 679–87 1268224 2 10.1016/0005-2795(76)90211-7 Rembeza E, Engqvist MK 2021 Experimental and computational investigation of enzyme functional annotations uncovers misannotation in the EC 1.1.3.15 enzyme class PLOS Comput Biol 17 e1009446 34555022 9 10.1371/journal.pcbi.1009446 8491902 free 2021PLSCB..17E9446R Biology no Category:EC 1.1.3 Category:Flavoproteins Category:Enzymes of known structure