(heparan sulfate)-glucosamine 3-sulfotransferase 1

Class of enzymes Heparin-glucosamine 3-O-sulfotransferase 2.8.2.23 118113-79-4 0008467 In enzymology, a [heparan sulfate]-glucosamine 3-sulfotransferase 1 (2.8.2.23) is an enzyme that catalyzes the chemical reaction 3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate Thus, the two substrates of this enzyme are 3'-phosphoadenylyl sulfate and heparan sulfate-glucosamine, whereas its two products are adenosine 3',5'-bisphosphate and heparan sulfate-glucosamine 3-sulfate. This enzyme belongs to the family of transferases, specifically the sulfotransferases, which transfer sulfur-containing groups. The systematic name of this enzyme class is 3'-phosphoadenylyl-sulfate:[heparan sulfate]-glucosamine 3-sulfotransferase. Other names in common use include heparin-glucosamine 3-O-sulfotransferase, 3'-phosphoadenylyl-sulfate:heparin-glucosamine 3-O-sulfotransferase, glucosaminyl 3-O-sulfotransferase, heparan sulfate D-glucosaminyl 3-O-sulfotransferase, and isoform/isozyme 1 (3-OST-1, HS3ST1). This enzyme participates in heparan sulfate biosynthesis and glycan structures - biosynthesis 1. Structural studies As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 1VKJ and 1ZRH. References 1 Kusche M, Backstrom G, Riesenfeld J, Petitou M, Choay J, Lindahl U 1988 Biosynthesis of heparin. O-sulfation of the antithrombin-binding region J. Biol. Chem. 263 15474–84 3139669 30 10.1016/S0021-9258(19)37613-6 free Shworak NW, Fritze LM, Liu J, Butler LD, Rosenberg RD 1996 Cell-free synthesis of anticoagulant heparan sulfate reveals a limiting converting activity that modifies an excess precursor pool J. Biol. Chem. 271 27063–27071 8900197 10.1074/jbc.271.43.27063 43 free Liu J, Shworak NW, Fritze LM, Edelberg JM, Rosenberg RD 1996 Purification of heparan sulfate D-glucosaminyl 3-O-sulfotransferase J. Biol. Chem. 271 27072–27082 8900198 10.1074/jbc.271.43.27072 43 free Rosenberg RD 1997 Molecular cloning and expression of mouse and human cDNAs encoding heparan sulfate D-glucosaminyl 3-O-sulfotransferase J. Biol. Chem. 272 28008–28019 9346953 10.1074/jbc.272.44.28008 Liu J Fritze LM Schwartz JJ Zhang L Logeart D Rosenberg RD 44 free Zhang L, Yoshida K, Liu J, Rosenberg RD 1999 Anticoagulant heparan sulfate precursor structures in F9 embryonal carcinoma cells J. Biol. Chem. 274 5681–5691 10026187 10.1074/jbc.274.9.5681 9 free RD 2001 The effect of precursor structures on the action of glucosaminyl 3-O-sulfotransferase-1 and the biosynthesis of anticoagulant heparan sulfate J. Biol. Chem. 276 28806–28813 11375390 10.1074/jbc.M100204200 Lawrence R Schwartz JJ Bai X Wei G Esko JD Rosenberg RD 31 free Biology no Category:EC 2.8.2 Category:Enzymes of known structure