2-methylcitrate synthase

Class of enzymes 2-methylcitrate synthase 2.3.3.5 57827-78-8 0050440 2-methylcitrate synthase (2.3.3.5) is an enzyme that catalyzes the chemical reaction 70% oxaloacetic acid + propionyl-CoA H2O Q105351054 (2R,3S)-2-methylcitric acid + coenzyme A The three substrates of this enzyme are oxaloacetic acid, propionyl-CoA, and water. Its two products are a specific stereoisomer of 2-methylcitric acid, with coenzyme A as the byproduct. The enyme has been characterised from Candida lipolytica, Uchiyama H Tabuchi T 1976 Properties of methylcitrate synthase from Candida lipolytica Agric. Biol. Chem. 40 7 1411–1418 10.1271/bbb1961.40.1411 free Escherichia coli, Buckel W 1997 Propionate oxidation in Escherichia coli: evidence for operation of a methylcitrate cycle in bacteria Arch. Microbiol. 168 428–36 9325432 10.1007/s002030050518 Wendisch VF De Graaf AA Müller U Linder MI Linder D Buckel W 5 1997ArMic.168..428T and Salmonella typhimurium.Horswill AR, Escalante-Semerena JC 1999 Salmonella typhimurium LT2 catabolizes propionate via the 2-methylcitric acid cycle J. Bacteriol. 181 5615–23 10482501 18 10.1128/JB.181.18.5615-5623.1999 94080 The isomer produced was shown to be (2R,3S)-2-methylcitric acid. Wadman SK 1994 Identification of the stereoisomeric configurations of methylcitric acid produced by si-citrate synthase and methylcitrate synthase using capillary gas chromatography-mass spectrometry J. Inherit. Metab. Dis. 17 738–47 7707698 10.1007/BF00712017 Mienie LJ Erasmus E De Wet WJ Ketting D Duran M Wadman SK 6 The methyl citrate product in fungi is later converted to methylisocitric acid and then to pyruvic acid and succinic acid by the enzyme methylisocitrate lyase.Domin Nicole Wilson Duncan Brock Matthias Methylcitrate cycle activation during adaptation of Fusarium solani and Fusarium verticillioides to propionyl-CoA-generating carbon sources Microbiology 2009 155 12 3903–3912 10.1099/mic.0.031781-0 free 19661181 Brock Matthias Maerker Claudia Schütz Alexandra Völker Uwe Buckel Wolfgang Oxidation of propionate to pyruvate in Escherichia coli European Journal of Biochemistry 2002 269 24 6184–6194 10.1046/j.1432-1033.2002.03336.x 12473114 This enzyme belongs to the family of transferases, specifically those acyltransferases that convert acyl groups into alkyl groups on transfer. The systematic name of this enzyme class is propanoyl-CoA:oxaloacetate C-propanoyltransferase (thioester-hydrolysing, 1-carboxyethyl-forming). Other names in common use include 2-methylcitrate oxaloacetate-lyase, MCS, methylcitrate synthase, and methylcitrate synthetase.2.3.3.5 References Biology no Category:EC 2.3.3 Category:Enzymes of unknown structure