16S rRNA (guanine1405-N7)-methyltransferase

Class of enzymes 16S rRNA (guanine1405-N7)-methyltransferase 2.1.1.179 16S rRNA (guanine1405-N7)-methyltransferase (2.1.1.179, methyltransferase Sgm, m7G1405 Mtase, Sgm Mtase, Sgm, sisomicin-gentamicin methyltransferase, sisomicin-gentamicin methylase, GrmA, RmtB, RmtC, ArmA) is an enzyme with systematic name S-adenosyl-L-methionine:16S rRNA (guanine1405-N7)-methyltransferase. Husain N, Tkaczuk KL, Tulsidas SR, Kaminska KH, Cubrilo S, Maravić-Vlahovicek G, Bujnicki JM, Sivaraman J Structural basis for the methylation of G1405 in 16S rRNA by aminoglycoside resistance methyltransferase Sgm from an antibiotic producer: a diversity of active sites in m7G methyltransferases Nucleic Acids Research 38 12 4120–32 July 2010 20194115 2896518 10.1093/nar/gkq122 Savic M, Lovric J, Tomic TI, Vasiljevic B, Conn GL Determination of the target nucleosides for members of two families of 16S rRNA methyltransferases that confer resistance to partially overlapping groups of aminoglycoside antibiotics Nucleic Acids Research 37 16 5420–31 September 2009 19589804 2760815 10.1093/nar/gkp575 Tomic TI, Moric I, Conn GL, Vasiljevic B Aminoglycoside resistance genes sgm and kgmB protect bacterial but not yeast small ribosomal subunits in vitro despite high conservation of the rRNA A-site Research in Microbiology 159 9–10 658–62 2008 18930134 2791848 10.1016/j.resmic.2008.09.006 Savic M, Ilic-Tomic T, Macmaster R, Vasiljevic B, Conn GL Critical residues for cofactor binding and catalytic activity in the aminoglycoside resistance methyltransferase Sgm Journal of Bacteriology 190 17 5855–61 September 2008 18586937 2519519 10.1128/jb.00076-08 Maravić Vlahovicek G, Cubrilo S, Tkaczuk KL, Bujnicki JM Modeling and experimental analyses reveal a two-domain structure and amino acids important for the activity of aminoglycoside resistance methyltransferase Sgm Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 1784 4 582–90 April 2008 18343347 10.1016/j.bbapap.2007.09.009 Kojic M, Topisirovic L, Vasiljevic B Cloning and characterization of an aminoglycoside resistance determinant from Micromonospora zionensis Journal of Bacteriology 174 23 7868–72 December 1992 10.1128/jb.174.23.7868-7872.1992 1447159 207509 Schmitt E, Galimand M, Panvert M, Courvalin P, Mechulam Y Structural bases for 16 S rRNA methylation catalyzed by ArmA and RmtB methyltransferases Journal of Molecular Biology 388 3 570–82 May 2009 19303884 10.1016/j.jmb.2009.03.034 Wachino J, Shibayama K, Kimura K, Yamane K, Suzuki S, Arakawa Y RmtC introduces G1405 methylation in 16S rRNA and confers high-level aminoglycoside resistance on Gram-positive microorganisms FEMS Microbiology Letters 311 1 56–60 October 2010 20722735 10.1111/j.1574-6968.2010.02068.x free Liou GF, Yoshizawa S, Courvalin P, Galimand M Aminoglycoside resistance by ArmA-mediated ribosomal 16S methylation in human bacterial pathogens Journal of Molecular Biology 359 2 358–64 June 2006 16626740 10.1016/j.jmb.2006.03.038 This enzyme catalyses the following chemical reaction S-adenosyl-L-methionine + guanine1405 in 16S rRNA S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA The enzyme specifically methylates guanine1405 at N7 in 16S rRNA. References External links 16S+rRNA+(guanine1405-N7)-methyltransferase Biology no Category:EC 2.1.1