(S)-2-haloacid dehalogenase

Class of enzymes (S)-2-haloacid dehalogenase 3.8.1.2 37289-39-7 0018784 In enzymology, a (S)-2-haloacid dehalogenase (3.8.1.2) is an enzyme that catalyzes the chemical reaction (S)-2-haloacid + H2O (R)-2-hydroxyacid + halide Thus, the two substrates of this enzyme are (S)-2-haloacid and H2O, whereas its two products are (R)-2-hydroxyacid and halide. This enzyme belongs to the family of hydrolases, specifically those acting on halide bonds in carbon-halide compounds. The systematic name of this enzyme class is (S)-2-haloacid halidohydrolase. Other names in common use include 2-haloacid dehalogenase[ambiguous], 2-haloacid halidohydrolase [ambiguous][ambiguous], 2-haloalkanoic acid dehalogenase, 2-haloalkanoid acid halidohydrolase, 2-halocarboxylic acid dehalogenase II, DL-2-haloacid dehalogenase[ambiguous], L-2-haloacid dehalogenase, and L-DEX. This enzyme participates in gamma-hexachlorocyclohexane degradation and 1,2-dichloroethane degradation. Structural studies As of late 2007, 10 structures have been solved for this class of enzymes, with PDB accession codes 1AQ6, 1JUD, 1QH9, 1QQ5, 1QQ6, 1QQ7, 1ZRM, 1ZRN, 2NO4, and 2NO5. References 1 Goldman P, Milne GW, Keister DB 1968 Carbon-halogen bond cleavage. 3. Studies on bacterial halidohrolases J. Biol. Chem. 243 428–34 5635785 2 10.1016/S0021-9258(18)99310-5 free Motosugi M, Esaki N, Soda K 1982 Preparation and properties of 2-halo acid dehalogenase from Pseudomonas putida Agric. Biol. Chem. 46 3 837–838 10.1271/bbb1961.46.837 free Klages U, Krauss S, Lingens F 1983 2-Haloacid dehalogenase from a 4-chlorobenzoate-degrading Pseudomonas spec. CBS 3 Hoppe-Seyler's Z. Physiol. Chem. 364 529–35 6873881 5 10.1515/bchm2.1983.364.1.529 Diez A, Prieto MI, Alvarez MJ, Bautista JM, Garrido J, Puyet A 1996 Improved catalytic performance of a 2-haloacid dehalogenase from Azotobacter sp. by ion-exchange immobilisation Biochem. Biophys. Res. Commun. 220 828–33 8607850 10.1006/bbrc.1996.0489 3 1996BBRC..220..828D Morsberger FM, Muller R, Otto MK, Lingens F, Kulbe KD 1991 Purification and characterization of 2-halocarboxylic acid dehalogenase II from Pseudomonas spec. CBS 3 Biol. Chem. Hoppe-Seyler 372 915–22 1772590 10 10.1515/bchm3.1991.372.2.915 Kohler R, Brokamp A, Schwarze R, Reiting RH, Schmidt FR 1998 Characteristics and DNA-sequence of a cryptic haloalkanoic acid dehalogenase from Agrobacterium tumefaciens RS5 Curr. Microbiol. 36 96–101 9425247 10.1007/s002849900286 2 12691244 Motosugi K, Esaki N, Soda K 1982 Bacterial assimilation of D- and L-2-chloropropionates and occurrence of a new dehalogenase Arch. Microbiol. 131 179–83 7103659 10.1007/BF00405875 3 1982ArMic.131..179M 35346002 Kurihara T, Esaki N, Soda K 2000 Bacterial 2-haloacid dehalogenases: structures and reaction mechanisms J. Mol. Catal., B Enzym. 10 1–3 57–65 10.1016/S1381-1177(00)00108-9 M, Tsunasawa S Esaki N 1996 Bacterial 2-haloacid dehalogenases: Structures and catalytic properties Pure Appl. Chem. 68 2097–2103 10.1351/pac199668112097 11 38522855 free Biology no Category:EC 3.8.1 Category:Enzymes of known structure