10-deacetylbaccatin III 10-O-acetyltransferase

Class of enzymes 10-deacetylbaccatin III 10-O-acetyltransferase 2.3.1.167 220946-63-4 0050643 10-deacetylbaccatin III 10-O-acetyltransferase (2.3.1.167) is an enzyme that catalyzes the chemical reaction 70% 10-Deacetylbaccatin 1.5 + class=skin-invert-image acetyl-CoA Baccatin III 1.5 + coenzyme A The enzyme completes the biosynthesis of baccatin III by transferring an acetyl group from acetyl-CoA to the intermediate 10-deacetylbaccatin, giving coenzyme A as a byproduct. It was isolated from yew trees (Genera Taxus) and the gene was expressed in Escherichia coli to allow characterisation.Walker K, Croteau R 2000 Molecular cloning of a 10-deacetylbaccatin III-10-O-acetyl transferase cDNA from Taxus and functional expression in Escherichia coli Proc. Natl. Acad. Sci. U.S.A. 97 583–7 10639122 10.1073/pnas.97.2.583 2 15373 2000PNAS...97..583W free It has also been expressed in the mushroom Flammulina velutipes.Han F, Kang LZ, Zeng XL, Ye ZW, Guo LQ, Lin JF Bioproduction of baccatin III, an advanced precursor of paclitaxol with transgenic Flammulina velutipes expressing 10-Deacetylbaccatin III-10-O-acetyl transferase gene. J Sci Food Agric 2014 94 12 2376–2383 10.1002/jsfa.6562 24403190 2014JSFA...94.2376H This enzyme belongs to the family of transferases, specifically those acyltransferases transferring groups other than aminoacyl groups. The systematic name of this enzyme class is acetyl-CoA:taxan-10beta-ol O-acetyltransferase.2.3.1.167 References Biology no Category:EC 2.3.1 Category:Enzymes of unknown structure