1,4-lactonase

Class of enzymes 1,4-lactonase 3.1.1.25 37278-38-9 0050490 1,4-lactonase (EC 3.1.1.25) is an enzyme that catalyzes the generic reaction a 1,4-lactone + H2O a 4-hydroxyacid For example, the enzyme characterised from human blood and rat liver microsomes gives the sugar acid, D-galactonic acid by hydrolyis of D-galactono-1,4-lactone:Fishbein WN, Bessman SP 1966 Purification and properties of an enzyme in human blood and rat liver microsomes catalyzing the formation and hydrolysis of γ-lactones. I. Tissue localization, stoichiometry, specificity, distinction from esterase J. Biol. Chem. 241 4835–41 4958984 21 10.1016/S0021-9258(18)99641-9 free Fishbein WN, Bessman SP 1966 Purification and properties of an enzyme in human blood and rat liver microsomes catalyzing the formation and hydrolysis of γ-lactones. II. Metal ion effects, kinetics, and equilibra J. Biol. Chem. 241 4842–7 4958985 21 10.1016/S0021-9258(18)99642-0 free Schomburg Dietmar Salzmann Margit Enzyme Handbook 3 1, 4-Lactonase 1991 107–109 10.1007/978-3-642-76463-9_21 978-3-642-48962-4 55% Q27098286 D-galactono-1,4-lactone H2O Q27101959 D-galactonic acid This enzyme is a hydrolase, specifically one acting on carboxylic ester bonds. The systematic name is 1,4-lactone hydroxyacylhydrolase. It is also called γ-lactonase. It requires calcium as Ca2+.3.1.1.25 Structural studies As of late 2007, three structures have been solved for this class of enzymes, with PDB accession codes 2DG0, 2DG1, and 2DSO. Applications A 1,4-lactonase was expressed in Escherichia coli and used as a highly efficient biocatalyst for asymmetric synthesis of chiral compounds.Chen Bing Fan Li-Qiang Xu Jian-He Zhao Jian Zhang Xian Ouyang Li-Ming October 2010 Biocatalytic properties of a recombinant Fusarium proliferatum lactonase with significantly enhanced production by optimal expression in Escherichia coli Applied Biochemistry and Biotechnology 162 3 744–756 10.1007/s12010-009-8819-1 1559-0291 19876606 References Biology no Category:EC 3.1.1 Category:Calcium enzymes Category:Enzymes of known structure