(S)-canadine synthase

Class of enzymes (S)-canadine synthase 1.14.19.68 114308-22-4 0047056 In enzymology, a (S)-canadine synthase (1.14.19.68) is an enzyme that catalyzes the chemical reaction (S)-tetrahydrocolumbamine + NADPH + H+ + O2 (S)-canadine + NADP+ + 2 H2O The 4 substrates of this enzyme are (S)-tetrahydrocolumbamine, NADPH, H+, and O2, whereas its 3 products are Canadine, NADP+, and H2O. This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and the other dehydrogenated. The systematic name of this enzyme class is (S)-tetrahydrocolumbamine,NADPH:oxygen oxidoreductase (methylenedioxy-bridge-forming). Other names in common use include (S)-tetrahydroberberine synthase, and (S)-tetrahydrocolumbamine oxidase (methylenedioxy-bridge-forming). This enzyme participates in alkaloid biosynthesis i. It employs one cofactor, heme-thiolate(P-450). References 1 Rueffer M, Zenk MH 1994 Canadine synthase from Thalictrum tuberosum cell cultures catalyses the formation of the methylenedioxy bridge in berberine synthesis Phytochemistry 36 1219–1223 10.1016/S0031-9422(00)89640-5 5 1994PChem..36.1219R http://nbn-resolving.de/urn:nbn:de:bvb:19-epub-3613-0 Biology no Category:EC 1.14.19 Category:NADPH-dependent enzymes Category:Heme enzymes Category:Enzymes of unknown structure