(2,2,3-Trimethyl-5-oxocyclopent-3-enyl)acetyl-CoA 1,5-monooxygenase

Class of enzymes (2,2,3-Trimethyl-5-oxocyclopent-3-enyl)acetyl-CoA 1,5-monooxygenase 1.14.13.160 (2,2,3-Trimethyl-5-oxocyclopent-3-enyl)acetyl-CoA 1,5-monooxygenase (1.14.13.160, 2-oxo-Delta3-4,5,5-trimethylcyclopentenylacetyl-CoA monooxygenase, 2-oxo-Delta3-4,5,5-trimethylcyclopentenylacetyl-CoA 1,2-monooxygenase, OTEMO) is an enzyme with systematic name ((1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl)acetyl-CoA,NADPH:oxygen oxidoreductase (1,5-lactonizing). Ougham HJ, Taylor DG, Trudgill PW Camphor revisited: involvement of a unique monooxygenase in metabolism of 2-oxo-delta 3-4,5,5-trimethylcyclopentenylacetic acid by Pseudomonas putida Journal of Bacteriology 153 1 140–52 January 1983 10.1128/jb.153.1.140-152.1983 6848481 217351 Leisch H, Shi R, Grosse S, Morley K, Bergeron H, Cygler M, Iwaki H, Hasegawa Y, Lau PC Cloning, Baeyer-Villiger biooxidations, and structures of the camphor pathway 2-oxo-Δ(3)-4,5,5-trimethylcyclopentenylacetyl-coenzyme A monooxygenase of Pseudomonas putida ATCC 17453 Applied and Environmental Microbiology 78 7 2200–12 April 2012 22267661 3302634 10.1128/AEM.07694-11 2012ApEnM..78.2200L Kadow M, Loschinski K, Sass S, Schmidt M, Bornscheuer UT Completing the series of BVMOs involved in camphor metabolism of Pseudomonas putida NCIMB 10007 by identification of the two missing genes, their functional expression in E. coli, and biochemical characterization Applied Microbiology and Biotechnology 96 2 419–29 October 2012 22286514 10.1007/s00253-011-3859-1 This enzyme catalyses the following chemical reaction [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetyl-CoA + O2 + NADPH + H+ [(2R)-3,3,4-trimethyl-6-oxo-3,6-dihydro-1H-pyran-2-yl]acetyl-CoA + NADP+ + H2O (2,2,3-trimethyl-5-oxocyclopent-3-enyl)acetyl-CoA 1,5-monooxygenase is FAD dependent enzyme isolated from Pseudomonas putida. References External links (2,2,3-trimethyl-5-oxocyclopent-3-enyl)acetyl-CoA+1,5-monooxygenase Biology no Category:EC 1.14.13